預(yù)染蛋白Ladders和Marker PageRuler Prest Protein Ladder
預(yù)染蛋白Ladders和Marker PageRuler Prest Protein Ladder
特點(diǎn)
預(yù)染蛋白Ladders和Marker PageRuler Prest Protein Ladder
應(yīng)用
說明
預(yù)染蛋白Ladders/marker設(shè)計(jì)用于監(jiān)控蛋白的SDS-聚丙烯酰胺凝膠電泳分離、驗(yàn)證Western時(shí)蛋白轉(zhuǎn)移至PVDF、尼龍和硝酸纖維膜的效率、粗略鑒定蛋白大小等應(yīng)用(1-3)。這些預(yù)染的蛋白分子量標(biāo)準(zhǔn)由預(yù)染的原核生物重組蛋白組成,是預(yù)染的天然蛋白的混合物。
PageRuler™ Prestained Protein Ladder是一 個(gè)三色分子量標(biāo)準(zhǔn),含有10種蛋白,分子量范圍為10 - 170 kDa。該分子量標(biāo)準(zhǔn)含有兩條參考條帶,分別為70 kDa的橙色參考條帶和10 kDa的綠色參考條帶。
保存緩沖液
62.5 mM Tris-H3PO4 (pH 7.5, 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3和33%甘油。
質(zhì)量控制
測試:Western雜交檢測和SDS-聚丙烯酰胺凝膠電泳。
保存
4°C穩(wěn)定保存3個(gè)月。如需保存更長時(shí)間,請-20°C保存。
圖1。Migration patterns of PageRuler™ Prestained Protein Ladder in different electrophoretic conditions.
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.
* Migration patterns were determined using respective NuPAGE® precast gels.
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